Anti-Human IL1 beta Recombinant Antibody (TAB-161)
CAT#: TAB-161
Recombinant monoclonal antibody to IL1 beta. This humanized monoclonal antibody is suitable for use in ELISA, FC, IP, FuncS, IF, Neut, ICC and most other immunological methods.
Gene Expression
Tested Data
RNA Expression
Figure 1 RNA cell line category: Cell line enhanced.
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000125538-IL1B
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SDS-PAGE
Figure 1 Anti-Human IL1 beta Recombinant Antibody (TAB-161) in SDS-PAGE
SDS-PAGE analysis of TAB-161 in reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
The purity of TAB-161 was greater than 95% as determined by SDS-PAGE.
SEC-HPLC
Figure 2 Anti-Human IL1 beta Recombinant Antibody (TAB-161) in HPLC
The purity of TAB-161 was greater than 95% as determined by SEC-HPLC.
WB
Figure 3 Anti-Human IL1 beta Recombinant Antibody (TAB-161) in WB
Western blot analysis of TAB-161 was performed by loading Recombinant Human IL1 beta protein.
TAB-161 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-human IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
ELISA
Figure 4 Anti-Human IL1 beta Recombinant Antibody (TAB-161) in ELISA
ELISA analysis of TAB-161 was performed by coating with Recombinant Human IL1 beta Protein. Then blocked with BSA and incubated with Anti-Human IL1 beta Recombinant Antibody (TAB-161). The Goat Anti-Human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Mouse
- Derivation
- Humanized (from mouse)
- Type
- IgG2 - kappa
- Specificity
- Tested positive against native human antigen.
- Species Reactivity
- Human
- Applications
- Suitable for use in ELISA, FC, IP, FuncS, IF, Neut, ICC and most other immunological methods.
- MW
- 145.2 kDa
- Related Disease
- Cardiovascular disease
Product Property
- Purity
- >95.0%. Determined by analysis by RP-HPLC & analysis by SDS-PAGE.
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Target
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(Creative Biolabs Cat# TAB-161, RRID: AB_3111842)
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Biosimilar Overview
Please refer to Gevokizumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Gevokizumab.
Related Diseases
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Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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